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bceB [2019-12-28 16:19:22]
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bceB [2019-12-28 16:19:22]

primary bacitracin resistance determinant, [SW|ABC transporter] (permease) for the export of bacitracin, plectasin, mersacidin and actagardine, sensory component of the Bce regulatory & detoxification system
locus
BSU_30370
pI
9.79
mw
72.01 kDa
protein length
646 aa Sequence Blast
gene length
1941 bp Sequence Blast
function
export of toxic peptides
product
bacitracin [SW|ABC transporter] (permease)
essential
no
synonyms
ytsD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.3|Export of peptides]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,109,397 → 3,111,337

    Phenotypes of a mutant

  • 85-fold increased sensitivity to bacitracin [Pubmed|26815905]

    • The protein

    Catalyzed reaction/ biological activity

  • binds bacitracin, KD: 60 nM [Pubmed|25118291]
  • inhibits [protein|08F24CA21DC54031F6158AC0C772F3B520E5A849|BceS] autophosphorylation activity in the absence of bacitracin [Pubmed|25118291]

    • Protein family

  • [SW|ABC-4 integral membrane protein family] (according to UniProt)

    • Effectors of protein activity

  • the activity of the [protein|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|BceA]-[protein|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|BceB] [SW|ABC transporter] is inhibited by heptaprenyl pyrophosphate which accumulates in a ''[gene|596C09DD1D9C305B7C49B2F5E90E2C6C33F63D0E|ytpB]'' mutant [Pubmed|24806199]

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|8E0DE8FE7E9C12495C4DCADA74D838FED088867D|bceA]-[gene|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB]
    description
    [Pubmed|12890034]

    regulatory mechanism

  • [protein|C239C155F5452C86BF6C78190BABBBB07A63DF87|BceR]: activation, [Pubmed|12890034], in [regulon|C239C155F5452C86BF6C78190BABBBB07A63DF87|BceR regulon]

    • regulation

  • induced in the presence of bacitracin, plectasin, mersacidin and actagardine, already at very low levels of bacitracin ([protein|search|BceR]) [Pubmed|12890034,26815905]
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    Biological materials

    Mutant

  • MGNA-A168 (ytsD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/168 NBRP B. subtilis, Japan]
  • BKE30370 (Δ[gene|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATTAATGTTCATGCTGCAC, downstream forward: _UP4_TGAAACAGAAAAAGCTCCAG
  • BKK30370 (Δ[gene|E3613A6C8B5F0EC76510ACE3E478967E73E2B241|bceB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATTAATGTTCATGCTGCAC, downstream forward: _UP4_TGAAACAGAAAAAGCTCCAG

    • References

    Reviews

  • 27344142,28152228,29404338

    • Original publications

  • 18394148,17905982,12890034,14651641,10092453,14612242,21283517,20606066,21078927,23687272,25118291,24806199,26199330,26815905